Re: Questions about culturing of NSC# Biology - 生物学
s*e
1 楼
It was perfect at first,at least for 4h. But after O/N, almost all died.
I'm 80% sure it's the coating problem.
I'm 80% sure it's the coating problem.
s*e
2 楼
I don't really know the answer.
But obviously cells are happier if they feel that they are in contact with
sth, either the dish or other cells.
I guess,
Cells in the dish are in an equilibrium between attach and detach,if the
coating is good,attaching wins out eventually. Otherwise detach win,in that
case cells will die because they feel so lonely.
Not all cells will die if the coating is not good, but most of them. You
can see that those who survive are mostly in aggregation, because by
aggrega
But obviously cells are happier if they feel that they are in contact with
sth, either the dish or other cells.
I guess,
Cells in the dish are in an equilibrium between attach and detach,if the
coating is good,attaching wins out eventually. Otherwise detach win,in that
case cells will die because they feel so lonely.
Not all cells will die if the coating is not good, but most of them. You
can see that those who survive are mostly in aggregation, because by
aggrega
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