yes. i did dialysis 500x twice to remove free labels. that's also what other people do after labeling.
biotin
【在 a*****n 的大作中提到】 : after labeling, did you get rid of the free biotin molecule? The free biotin : will compete with the labeled protein and it is more abundant.
c*u
16 楼
找个过期的antibody 作positive test吧。 NHS 是标抗体最常用的办法。
other
【在 n********e 的大作中提到】 : yes. i did dialysis 500x twice to remove free labels. that's also what other : people do after labeling. : : biotin
Octec is better than biacore. You could add avi tag to your protein DNA sequence and express it in the mammalian cells(293T). Octec company have the biotin tips, and you can purify the protein and use the biotin tips directly. dont put biotin into your cells. It would be copurified with your avitag protein and interfere the following binding assay.