w*o
2 楼
IRS website says Jan 15 is the last day for the 4th quater payment.
why the payment centers like these: http://www.irs.gov/uac/Pay-Taxes-by-Credit-or-Debit-Card
can take the payment till Feb 01?
why the payment centers like these: http://www.irs.gov/uac/Pay-Taxes-by-Credit-or-Debit-Card
can take the payment till Feb 01?
z*e
3 楼
打算签德国,情况如下,不知能否签过:F1的VISA早过期了,换了H1B以后没有回去返签
过,打算去欧洲玩2个月,然后回国,美国的工作不做了,之后暂定不回美。护照还有7
个月过期,打算明年5-6月去玩,有必要换护照吗,何时去签证好?谢谢
过,打算去欧洲玩2个月,然后回国,美国的工作不做了,之后暂定不回美。护照还有7
个月过期,打算明年5-6月去玩,有必要换护照吗,何时去签证好?谢谢
U*n
4 楼
朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
首音乐,
非常真实反映了宗教的庄严,雄浑和肃穆。
下载地址:
http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
首音乐,
非常真实反映了宗教的庄严,雄浑和肃穆。
下载地址:
http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
q*n
5 楼
If I have two genes, A and B. Using real time RT-PCR, in the same cell lines
, the Ct value of A is about 22, and the ct value of B is about 28. 每个基因
各用两套primer sets, 都得到相同的结果。我能说 基因A表达量比B高吗?
有没有网站讲这个的呀?
谢谢
, the Ct value of A is about 22, and the ct value of B is about 28. 每个基因
各用两套primer sets, 都得到相同的结果。我能说 基因A表达量比B高吗?
有没有网站讲这个的呀?
谢谢
C*t
6 楼
若这个孩子不具有中国国籍的话,最多24小时内须登记。
依照《中国出入境管理法》第三十九条——
外国人在中国境内旅馆住宿的,旅馆应当按照旅馆业治安管理的有关规定为其办理住宿
登记,并向所在地公安机关报送外国人住宿登记信息。外国人在旅馆以外的其他住所居
住或者住宿的,应当在入住后二十四小时内由本人或者留宿人,向居住地的公安机关办
理登记。
依照《中国出入境管理法》第三十九条——
外国人在中国境内旅馆住宿的,旅馆应当按照旅馆业治安管理的有关规定为其办理住宿
登记,并向所在地公安机关报送外国人住宿登记信息。外国人在旅馆以外的其他住所居
住或者住宿的,应当在入住后二十四小时内由本人或者留宿人,向居住地的公安机关办
理登记。
N*g
7 楼
1/31 is the last day if you owe nothing to IRS (i.e. you will get a tax
refund, not mailing IRS a check in your tax return).
http://www.irs.gov/publications/p505/ch02.html
January payment. If you file your 2012 Form 1040 or Form 1040A by January
31, 2013, and pay the rest of the tax you owe, you do not need to make the
payment due on January 15, 2013.
Example.
Janet Adams does not pay any estimated tax for 2012. She files her 2012
income tax return and pays the balance due shown on her return on January 26
, 2013.
Janet's estimated tax for the fourth payment period is considered to have
been paid on time. However, she may owe a penalty for not making the first
three estimated tax payments, if required. Any penalty for not making those
payments will be figured up to January 26, 2013.
http://www.yeoandyeo.com/uploads/2013%20Individuals%20Trusts%20
refund, not mailing IRS a check in your tax return).
http://www.irs.gov/publications/p505/ch02.html
January payment. If you file your 2012 Form 1040 or Form 1040A by January
31, 2013, and pay the rest of the tax you owe, you do not need to make the
payment due on January 15, 2013.
Example.
Janet Adams does not pay any estimated tax for 2012. She files her 2012
income tax return and pays the balance due shown on her return on January 26
, 2013.
Janet's estimated tax for the fourth payment period is considered to have
been paid on time. However, she may owe a penalty for not making the first
three estimated tax payments, if required. Any penalty for not making those
payments will be figured up to January 26, 2013.
http://www.yeoandyeo.com/uploads/2013%20Individuals%20Trusts%20
m*a
8 楼
18
Thank you so much!
I will download it tonight!---I like the title--God is our heavenly father!
【在 U***n 的大作中提到】
: 朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
: 首音乐,
: 非常真实反映了宗教的庄严,雄浑和肃穆。
: 下载地址:
: http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
w*e
9 楼
No, you can't. The expression of gene of interest has to be compared against
that of house-keeping genes.
that of house-keeping genes.
w*o
10 楼
是不是只要2012年交的税比2011年多就没有罚款,即使你欠IRS税。
January
26
【在 N****g 的大作中提到】
: 1/31 is the last day if you owe nothing to IRS (i.e. you will get a tax
: refund, not mailing IRS a check in your tax return).
: http://www.irs.gov/publications/p505/ch02.html
: January payment. If you file your 2012 Form 1040 or Form 1040A by January
: 31, 2013, and pay the rest of the tax you owe, you do not need to make the
: payment due on January 15, 2013.
: Example.
: Janet Adams does not pay any estimated tax for 2012. She files her 2012
: income tax return and pays the balance due shown on her return on January 26
: , 2013.
January
26
【在 N****g 的大作中提到】
: 1/31 is the last day if you owe nothing to IRS (i.e. you will get a tax
: refund, not mailing IRS a check in your tax return).
: http://www.irs.gov/publications/p505/ch02.html
: January payment. If you file your 2012 Form 1040 or Form 1040A by January
: 31, 2013, and pay the rest of the tax you owe, you do not need to make the
: payment due on January 15, 2013.
: Example.
: Janet Adams does not pay any estimated tax for 2012. She files her 2012
: income tax return and pays the balance due shown on her return on January 26
: , 2013.
A*e
11 楼
下ING.不好听的话拿臭鸡蛋打你 :)
18
【在 U***n 的大作中提到】
: 朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
: 首音乐,
: 非常真实反映了宗教的庄严,雄浑和肃穆。
: 下载地址:
: http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
18
【在 U***n 的大作中提到】
: 朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
: 首音乐,
: 非常真实反映了宗教的庄严,雄浑和肃穆。
: 下载地址:
: http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
t*t
12 楼
蛋白水平是可以比较的, RNA的不知道.
应该也可以吧. 但得先设一系列的梯度, 可能要对2个基因各设一套. 然后根据PCR产物的量推断RNA
的绝对含量.
应该也可以吧. 但得先设一系列的梯度, 可能要对2个基因各设一套. 然后根据PCR产物的量推断RNA
的绝对含量.
u*8
14 楼
我觉得应该可以比,但是我们一般不这样比,我们只是比同各基因,在不同sample中的表
达差异.
不同的基因,即使有差异,又怎么解释呢?有些基因,即使增加1倍,也会有很大的效应.相
对另外一些基因,即使增加很多倍,效应也很小.所以我觉得主要看你的实验目的,看对比
这种差异是否有意义.
不知道我的想法对不对
达差异.
不同的基因,即使有差异,又怎么解释呢?有些基因,即使增加1倍,也会有很大的效应.相
对另外一些基因,即使增加很多倍,效应也很小.所以我觉得主要看你的实验目的,看对比
这种差异是否有意义.
不知道我的想法对不对
m*a
15 楼
18
我正在听,很好啊,谢谢分享。
【在 U***n 的大作中提到】
: 朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
: 首音乐,
: 非常真实反映了宗教的庄严,雄浑和肃穆。
: 下载地址:
: http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
w*d
18 楼
in theory, it is really painful to compare different genes transcript
expression level by qPCR. you have to go down to the "absolute" number of
transcript. in this situation, relative fold doesn't work. you have to make
known copy number of genes as standard. even after that, you can't be sure
that in your system that PCR efficiency for different sets of primers would
behave the same.
I would say if your difference is kind of dramatic (in relative method), you
would be confident to show the diff
expression level by qPCR. you have to go down to the "absolute" number of
transcript. in this situation, relative fold doesn't work. you have to make
known copy number of genes as standard. even after that, you can't be sure
that in your system that PCR efficiency for different sets of primers would
behave the same.
I would say if your difference is kind of dramatic (in relative method), you
would be confident to show the diff
E*1
19 楼
我们要看生活片!!!
【在 m****a 的大作中提到】
: http://www.youtube.com/watch?v=-0Gj8-vB1q4&feature=related
【在 m****a 的大作中提到】
: http://www.youtube.com/watch?v=-0Gj8-vB1q4&feature=related
f*r
20 楼
“表达量”是个什么概念?
如果是同一基因,在不同samples里(或者说不同条件下),表达有量化的差异,那么
RT-PCR完全可以说明问题。因为除转录之外的其它环节是恒定的。
不同基因,即使在同一sample里,即使mRNA产量有区别,又能说明什么?毕竟翻译、修
饰、激活、生物学活性的调节、失活、降解的机制与效率不同。
但是,如果仅仅想表示两个基因的转录数量有差异,你的数据就可以用。当然,你必须
证明做RT-PCR的时候,两套基因的逆转录效率和PCR扩增效率是相同的或者可比的。
如果是同一基因,在不同samples里(或者说不同条件下),表达有量化的差异,那么
RT-PCR完全可以说明问题。因为除转录之外的其它环节是恒定的。
不同基因,即使在同一sample里,即使mRNA产量有区别,又能说明什么?毕竟翻译、修
饰、激活、生物学活性的调节、失活、降解的机制与效率不同。
但是,如果仅仅想表示两个基因的转录数量有差异,你的数据就可以用。当然,你必须
证明做RT-PCR的时候,两套基因的逆转录效率和PCR扩增效率是相同的或者可比的。
m*a
21 楼
18
才发现这张CD似乎都是摩门教诗歌本里的呢。。
【在 U***n 的大作中提到】
: 朋友给的,可能是他自己以前录广播,然后烧CD给我的。管弦乐,制作水准非常高。18
: 首音乐,
: 非常真实反映了宗教的庄严,雄浑和肃穆。
: 下载地址:
: http://uploading.com/files/c145ebe3/O%2BGod%2BMy%2BFather.rar/
K*y
22 楼
歌很好听,不过我不是基督徒,呵呵
【在 m****a 的大作中提到】
: http://www.youtube.com/watch?v=-0Gj8-vB1q4&feature=related
【在 m****a 的大作中提到】
: http://www.youtube.com/watch?v=-0Gj8-vB1q4&feature=related
m*a
24 楼
这首我也很喜欢:
http://www.youtube.com/watch?v=jW5bKEc8hQg
I am a child of God,
And he has sent me here,
Has given me an earthly home
With parents kind and dear.
Lead me, guide me, walk beside me,
Help me find the way.
Teach me all that I must do
To live with him someday.
I am a child of God,
And so my needs are great;
Help me to understand his words
Before it grows too late.
I am a child of God.
Rich blessings are in store;
If I but learn to
http://www.youtube.com/watch?v=jW5bKEc8hQg
I am a child of God,
And he has sent me here,
Has given me an earthly home
With parents kind and dear.
Lead me, guide me, walk beside me,
Help me find the way.
Teach me all that I must do
To live with him someday.
I am a child of God,
And so my needs are great;
Help me to understand his words
Before it grows too late.
I am a child of God.
Rich blessings are in store;
If I but learn to
K*y
25 楼
我曾经在这里混过的,觉得信仰这个东西越辩论越坚持自己的立场
信仰是解决心灵问题的,信了就信了,也没什么逻辑,
我以前常去教会玩,就是去唱诗弹吉他玩的,现在也去的,我过去比较极端,现在比较
温和,呵呵
【在 m****a 的大作中提到】
: 这首我也很喜欢:
: http://www.youtube.com/watch?v=jW5bKEc8hQg
: I am a child of God,
: And he has sent me here,
: Has given me an earthly home
: With parents kind and dear.
: Lead me, guide me, walk beside me,
: Help me find the way.
: Teach me all that I must do
: To live with him someday.
信仰是解决心灵问题的,信了就信了,也没什么逻辑,
我以前常去教会玩,就是去唱诗弹吉他玩的,现在也去的,我过去比较极端,现在比较
温和,呵呵
【在 m****a 的大作中提到】
: 这首我也很喜欢:
: http://www.youtube.com/watch?v=jW5bKEc8hQg
: I am a child of God,
: And he has sent me here,
: Has given me an earthly home
: With parents kind and dear.
: Lead me, guide me, walk beside me,
: Help me find the way.
: Teach me all that I must do
: To live with him someday.
K*y
26 楼
我也想有个什么信仰,似乎很难,但是我只有不极端就好了
K*y
27 楼
我喜欢这首,因为我会唱,呵呵
AMAZING GRACE
不同的版本
http://www.youtube.com/watch?v=HsCp5LG_zNE&feature=related
http://www.youtube.com/watch?v=GYMLMj-SibU
AMAZING GRACE
不同的版本
http://www.youtube.com/watch?v=HsCp5LG_zNE&feature=related
http://www.youtube.com/watch?v=GYMLMj-SibU
m*a
29 楼
我也很喜欢这首歌。
【在 K*******y 的大作中提到】
: 我喜欢这首,因为我会唱,呵呵
: AMAZING GRACE
: 不同的版本
: http://www.youtube.com/watch?v=HsCp5LG_zNE&feature=related
: http://www.youtube.com/watch?v=GYMLMj-SibU
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