请教精确定量NH2 group的方法# Biology - 生物学
w*e
1 楼
要在RNA synthesis的同时在5'end加NH2 group，不知道有没有什么精确定量方法，以便
确定NH2 incorporation efficiency。 Thank you!
确定NH2 incorporation efficiency。 Thank you!
a*o
2 楼
Is it possible to synthesize a short RNA oligo with NH2 at 5', anneal this
oligo to your single strand DNA template, add T7 RNA polymerase to
transcribe. The yield may be low, but the incorporation efficiency should be
really high. :)))
oligo to your single strand DNA template, add T7 RNA polymerase to
transcribe. The yield may be low, but the incorporation efficiency should be
really high. :)))
w*e
3 楼
I didn't know you can do that... so let me confirm: you mean, by anneal
a RNA oligo to a ssDNA template to make partial strand, the T7 polymerase
actually synthesize RNA by adding NTP to the existing RNA oligo, kinda
like a primer?
be
【在 a****o 的大作中提到】
: Is it possible to synthesize a short RNA oligo with NH2 at 5', anneal this
: oligo to your single strand DNA template, add T7 RNA polymerase to
: transcribe. The yield may be low, but the incorporation efficiency should be
: really high. :)))
a RNA oligo to a ssDNA template to make partial strand, the T7 polymerase
actually synthesize RNA by adding NTP to the existing RNA oligo, kinda
like a primer?
be
【在 a****o 的大作中提到】
: Is it possible to synthesize a short RNA oligo with NH2 at 5', anneal this
: oligo to your single strand DNA template, add T7 RNA polymerase to
: transcribe. The yield may be low, but the incorporation efficiency should be
: really high. :)))
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