s*e
2 楼
从来没有用过HUVEC primary cells, 大家用的都是哪个公司的啊?
我看到网上这种讨论很多,似乎推荐的都是Lonza的Clonetics的细胞,看到一些讨论说
ATCC的不好。
https://shop.lonza.com/shop/prd/human-umbilical-vein-endothelial-cells/lonza
_b2b/7.0-7_2_86_69_76_10_13/2/DF3699839AD6E6F18852001A4B525E10/
还有一个问题,我拿到了cells,可以生长细胞之后,做一大批的liquid nitrogen
frozen stock,以后的实验就用液氮里面冻着的细胞吗?
非常谢谢
我看到网上这种讨论很多,似乎推荐的都是Lonza的Clonetics的细胞,看到一些讨论说
ATCC的不好。
https://shop.lonza.com/shop/prd/human-umbilical-vein-endothelial-cells/lonza
_b2b/7.0-7_2_86_69_76_10_13/2/DF3699839AD6E6F18852001A4B525E10/
还有一个问题,我拿到了cells,可以生长细胞之后,做一大批的liquid nitrogen
frozen stock,以后的实验就用液氮里面冻着的细胞吗?
非常谢谢
i*g
3 楼
co-Ask HUVEC 哪种培养液最好?
w*w
4 楼
we use HUVEC from Lonza and buy medium from them too. you can freeze them
and use them. however, don't use them when you pass them over 9 times.
and use them. however, don't use them when you pass them over 9 times.
m*7
5 楼
We get both pooled HUVECs and medium (EGM-2) from Lonza.
For my specific purposes, I only use HUVECs within 5 passages.
When you propagate the primary stock, grow up as many cells and freeze down
as many vials of passage 2 as possible.
For my specific purposes, I only use HUVECs within 5 passages.
When you propagate the primary stock, grow up as many cells and freeze down
as many vials of passage 2 as possible.
x*a
6 楼
Lonza好像只保证15个doubling。你买的amp是passage 3,一般我只用到passage 7,
passage 8应该也没问题。有的实验室用到passage 10。
我曾经用flow cytometry测了下CD31,大约75%的positive吧。
passage 8应该也没问题。有的实验室用到passage 10。
我曾经用flow cytometry测了下CD31,大约75%的positive吧。
s*e
7 楼
非常谢谢
i*g
8 楼
非常谢谢
相关阅读
pBigT cloning学生物/化学的真可怕一个公司可以同时申两个Intern吗?搞aptamer/tumor imaging的人看了湖南大学的pnas了没?如果只盯着一个蛋白,该怎么研究才能发好文章?关于Western的蛋白定量。加州理工破NCAA26年连败史 两中国球员助校翻身 (转载)BPRC 北京蛋白质组研究中心求一篇文章,非常急谢谢当请人过来做seminar遇到环保主义者。。。PLoS One的文章有些还是很不错的,看这篇--zz隔壁实验室研究生invitrogen iblot system + Roche Dig labeling + IRDYE 800 southern blot招博士后啦求文献一篇, 非常感谢Do I have to pay the H1b extension fee by myself?博后面试之后隔多久follow up?学术海龟的烦恼 (转载)怎么能知道老板有没有funding?Nature Medicine presubmission inquiry