看过该网站回复人的帖子后,发现原来造假问题是异常严重的:WB里面,GAPDH的一张
图片被反复用在多个图中;而且在他们后来发表的其它 8 篇论文中,还存在相同的问
题!这是很明显的蓄意作假了。这些论文在下面用黑体标明:
7 Responses:
Well that was easy….In the v282#17p12363 paper, look at the GAPDH bands in
Figure 3A. Now go back to Figure 2C and look at the GAPDH bands. Lanes 4-5-6
are the same. They duplicated the blot.In the v283#29p20045 paper, Fig 3A
right panel GAPDH blot contains the same 2 bands as Fig. 5D.So, the “
mistake” appears to involve using the same GAPDH blot for different
experiments. This is so common, it’s not even funny any more!
VirgilAugust 8, 2011 at 10:12 amReplyYep, totally agree. I was immediately
drawn to the GAPDH blots in both papers. I have a feeling there is more
duplication in the other blots just using the famous “eyeball test”, but
have not had time to really look at them yet. I also agree that it is not
even funny any more and JBC is the worst for this kind of stuff. Why are
they so arrogant with the retraction notices? They should come out with it.
The journal is in a steep decline anyway and i really don’t trust the
majority of articles in it anymore. Shame.DaveAugust 8, 2011 at 2:01
pmReplyI’m no Photoshop expert, but in Vol 283, the GAPDH blot in Fig 2D (
lower right of panel) looks completely fabricated. When you zoom in, the
pixels just look completely different to the other blots and the shape and
density of the bands looks suspicious to me. I could be wrong.DaveAugust 8,
2011 at 2:12 pmReplyThey loved that figure so much, they published it more
than twice ! The HCN4 and GAPDH blots in Figure 3a of Luo et al., J Biol
Chem 283 (29):20045, 2009 are also the same as those in Figure 4a of Lin et
al., Cell Physiol Biochem 23:317-326, 2009. Things like this usually hint at
bigger problems. Other dubious data from the Wang lab include:The Nkx3.1
EMSA in Figure 6c of Lin et al., J Cell Physiol 212:137, 2007 is the same as
the Stat3 EMSA in Figure 5a of Gao et al., Mol Pharmacol 70:1621, 2006The
E2F1 ChIP blot in Figure 1d of Lin et al., PloSOne 6(5):e20362, 2011 is the
same as the p53 CHiP blot in Figure 3a of Xiao et al., EMBO J 30:524,
2011Lanes 3-6 of the GAPDH control for the HSP70 blot in Figure 3b of Xu et
al., J Cell Sci 120(17): 3045-3052, 2007 are the same as the GAPDH control
for the Nkx3.1 blot in Figure 6g of Lin et al., J Cell Physiol, 212:137,
2007“Oh what a tangled web we weave…”