【在 g*****9 的大作中提到】 : Is 5% of the house price more than $5000 ?
c*g
11 楼
Maybe NP40 or charps is better for COIP of membrane proteins。 RIPA lysis buffer裂cells这一点上应该问题不大:我们的做法是加上RIPA,然后用 1ml针管+#7针头needle 10 times;最高速离心10min @4oC后去上清即可。
g*9
12 楼
I think you should be ok.
【在 o****n 的大作中提到】 : yes
s*e
13 楼
to extract protein from membrane, you need to allow a 0.5-1h incubation& shaking time. If you worried that NP40 is not good enough to solubilize lipid membrane, an additional detergent such as DDM could be added into your lysis buffer.
【在 s*******e 的大作中提到】 : to extract protein from membrane, you need to allow a 0.5-1h incubation& : shaking time. If you worried that NP40 is not good enough to solubilize : lipid membrane, an additional detergent such as DDM could be added into your : lysis buffer.
s*d
18 楼
说明你的价出的太高了。
r*y
19 楼
去还是取?
【在 c****g 的大作中提到】 : Maybe NP40 or charps is better for COIP of membrane proteins。 : RIPA lysis buffer裂cells这一点上应该问题不大:我们的做法是加上RIPA,然后用 : 1ml针管+#7针头needle 10 times;最高速离心10min @4oC后去上清即可。
w*y
20 楼
ft, 在南加出的不高能抢到嘛?
【在 s**********d 的大作中提到】 : 说明你的价出的太高了。
D*9
21 楼
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