depends on how well you want to finish your tissue, which may affect your following procedures like IHC. I have never done on livers. What I may do, is to perfuse with 4% PFA and post-fix in 4% PFA for a bit longer.
O*C
10 楼
1000 max now.
o*r
11 楼
顶一下。
t*n
12 楼
4%PFA overnight/shaking/4 degree Liver tissue may need further cuts to help infiltration.