most likely it is NcoI site CCATGG andw eGFP starts with ATGG. addgene has a ton of plasmids with IRES-eGFP, i would buy one and take out the whole fragment to avoid any trouble.
RE LZ here you go Plasmid Information Toolbox-No. 114 Functional Description Retroviral expression vector derives from pMIG; the GFP of the original IRES-GFP casette is exchanged with the enhanced cyan flourescent protein. . The vector allows cloning of genes of interest on the N-terminus of IRES for co-expression with CFP in cells. web link: http://www.bioss.uni-freiburg.de/toolbox/products.php?PL-62 then pls send mail to German side for it: link: http://www.bioss.uni-freiburg.de/toolbox/info.php?contact&osCsi Ps: LZ 有包子 就给个
you can, but you also have to be careful, the distance between the IRES and the ATG is very important, which will influence the expression of the following gene. therefore, you'd better use the same amount of bases as that in the original vector.
【在 p*****e 的大作中提到】 : you can, but you also have to be careful, the distance between the IRES and : the ATG is very important, which will influence the expression of the : following gene. therefore, you'd better use the same amount of bases as that : in the original vector. : : GFP