2nd this. The amplification efficiency of Taq drops sharply when the amplified fragment is more than 6-7 kb. Phusion works ok for 10 kb, plus it is fast, saves a lot of time.
Thanks for the above sharing. Not sure how high the incorporate error rate of phusion or PfuUltra high-fidelity DNA polymerase. Do both of them have high-fidelity amplification? Thanks.
d*1
16 楼
我根本没有预测:我是说才过半年。这些数据还都没有结果。。
【在 n****o 的大作中提到】 : 你这个预测是你几乎所有悲观预测中难见的乐观预测
o*l
17 楼
小p太可怜了 阿姨抱抱
【在 h******n 的大作中提到】 : 广发英雄帖,潦倒颓废派的来PK吧 ^O^
q*0
18 楼
Which company's phusion is better, Thermo Scientific or New England Biolab? Thanks.
【在 q**********0 的大作中提到】 : Thanks for the above sharing. Not sure how high the incorporate error rate : of phusion or PfuUltra high-fidelity DNA polymerase. Do both of them have : high-fidelity amplification? Thanks.
h*n
22 楼
帕里黯然的说:‘我终于领悟独孤求败的孤独了’~~~ (背景是秋风萧瑟,树叶狂 飞。。。)
【在 f*********n 的大作中提到】 : 哈哈哈哈,这个比较难。。。
s*s
23 楼
只用过thermo的,neb也出phusion了?
?
【在 q**********0 的大作中提到】 : Which company's phusion is better, Thermo Scientific or New England Biolab? : Thanks.
h*n
24 楼
我使劲的蹭,阿姨胸前湿掉一大片。(脸红的跑开~~~)
【在 o********l 的大作中提到】 : 小p太可怜了 : 阿姨抱抱
q*0
25 楼
Yes, NEB also has phusion, but they said theirs is from thermo.