e*t
2 楼
刚买的nexus model是 GT-i9250m,哪位大侠说说下面这个case能不能用?
http://www.amazon.com/Black-Androidified-Samsung-SCH-i515-GT-i9
http://www.amazon.com/Black-Androidified-Samsung-SCH-i515-GT-i9
t*k
3 楼
之前做ChIP-qPCR的primer都是用别人已经发表的,然后今天听人说在不同细胞组织里
面同一个基因的promoter会不一样,所以最好用细胞特异的primer。我之前用的primer
都是别人发表的es细胞里面,但我作的不是es。于是开始对之前自己做的结果开始怀疑
了。。。。。大家有什么建议的么~~~~
面同一个基因的promoter会不一样,所以最好用细胞特异的primer。我之前用的primer
都是别人发表的es细胞里面,但我作的不是es。于是开始对之前自己做的结果开始怀疑
了。。。。。大家有什么建议的么~~~~
d*g
4 楼
It's 30k if i leave the company in a year after green
e*t
5 楼
Product Description
CruzerLite Galaxy Nexus Cases fit all 3 versions of the Galaxy Nexus (Sprint
, Verizon, GSM)
CruzerLite Galaxy Nexus Cases fit all 3 versions of the Galaxy Nexus (Sprint
, Verizon, GSM)
D*g
6 楼
It is true that some genes have alternative promoters for different cells/
tissues, however, the primers for ChIP-qPCR should cover the binding site of
your target transcription factor, therefore you should not worry about your
primers. If you know potential binding site of your target transcription
factor in other potential promoter of your gene, probably you should design
primers to cover those binding sites.
tissues, however, the primers for ChIP-qPCR should cover the binding site of
your target transcription factor, therefore you should not worry about your
primers. If you know potential binding site of your target transcription
factor in other potential promoter of your gene, probably you should design
primers to cover those binding sites.
l*8
7 楼
听律师说律师费一定要公司出,自己出,被查出来要坐牢,有这么严重吗?
e*t
8 楼
打开wifi,玩会游戏,都会有这个现象。
K*R
9 楼
主要第一是看看你测试什么的binding, 比如转录因子TFs 还是histone的。
设计primer 比较麻烦,
首先看promoter位置,看有人报道过没有。 如果没有就预测,
主要是具体选择哪个区域,有时候不一定严格选promoter, promoter附近也可以。
根据你要pull down 蛋白来定
看看Dr young 的设计方法,希望你能用的上。
http://younglab.wi.mit.edu/regulatory_code/
设计primer 比较麻烦,
首先看promoter位置,看有人报道过没有。 如果没有就预测,
主要是具体选择哪个区域,有时候不一定严格选promoter, promoter附近也可以。
根据你要pull down 蛋白来定
看看Dr young 的设计方法,希望你能用的上。
http://younglab.wi.mit.edu/regulatory_code/
r*d
12 楼
thx for sharing
f*r
13 楼
is it ok to reimburse the fee later to employer?
t*k
15 楼
看样子应该问题不大吧,谢谢哈~~
of
your
design
【在 D****g 的大作中提到】
: It is true that some genes have alternative promoters for different cells/
: tissues, however, the primers for ChIP-qPCR should cover the binding site of
: your target transcription factor, therefore you should not worry about your
: primers. If you know potential binding site of your target transcription
: factor in other potential promoter of your gene, probably you should design
: primers to cover those binding sites.
of
your
design
【在 D****g 的大作中提到】
: It is true that some genes have alternative promoters for different cells/
: tissues, however, the primers for ChIP-qPCR should cover the binding site of
: your target transcription factor, therefore you should not worry about your
: primers. If you know potential binding site of your target transcription
: factor in other potential promoter of your gene, probably you should design
: primers to cover those binding sites.
m*u
16 楼
瞎说,我在学校里,都自己出,报税的时候可以免税而已
H1b的倒是学校出
H1b的倒是学校出
t*k
17 楼
是histone,就是因为觉得设计这个的primer很麻烦,看有些文章对一个基因都要试n对
引物,所以就想走捷径用别人发表的。
【在 K**R 的大作中提到】
: 主要第一是看看你测试什么的binding, 比如转录因子TFs 还是histone的。
: 设计primer 比较麻烦,
: 首先看promoter位置,看有人报道过没有。 如果没有就预测,
: 主要是具体选择哪个区域,有时候不一定严格选promoter, promoter附近也可以。
: 根据你要pull down 蛋白来定
: 看看Dr young 的设计方法,希望你能用的上。
: http://younglab.wi.mit.edu/regulatory_code/
引物,所以就想走捷径用别人发表的。
【在 K**R 的大作中提到】
: 主要第一是看看你测试什么的binding, 比如转录因子TFs 还是histone的。
: 设计primer 比较麻烦,
: 首先看promoter位置,看有人报道过没有。 如果没有就预测,
: 主要是具体选择哪个区域,有时候不一定严格选promoter, promoter附近也可以。
: 根据你要pull down 蛋白来定
: 看看Dr young 的设计方法,希望你能用的上。
: http://younglab.wi.mit.edu/regulatory_code/
m*u
20 楼
报税的时候有一项legal fee啥的,我就都填上去了
l*8
22 楼
Does anyone reimburse green card cost to employer?
d*g
23 楼
It's 30k if i leave the company in a year after green
l*8
24 楼
听律师说律师费一定要公司出,自己出,被查出来要坐牢,有这么严重吗?
f*r
26 楼
is it ok to reimburse the fee later to employer?
m*u
27 楼
瞎说,我在学校里,都自己出,报税的时候可以免税而已
H1b的倒是学校出
H1b的倒是学校出
m*u
30 楼
报税的时候有一项legal fee啥的,我就都填上去了
p*l
33 楼
PERM和140是以单位名义申请的,一定需要单位给付,但涉及的律师费是可以个人支付的
485好像是可以个人支付
485好像是可以个人支付
L*n
34 楼
Yes, PERM 一定需要单位给付, 但 140, 485 可以个人支付. See link below:
http://counsel.cua.edu/fedlaw/lpr.cfm
1. PERM (Labor Certification Application): Under the DOL's new rules, the
employer (i.e., college) must bear the costs associated with the preparation
and filing of a PERM application with the DOL including, but not limited to
, the advertising costs and attorney's fees (in most situations).
2. I-140 Immigration Petition for Alien Worker: There is no legal obligation
for the employer to pay any costs associated with the preparation and
filing of an I-140 with the USCIS.
3. I-485 Application to Adjust Status to Permanent Residence: There is no
legal obligation for the employer to pay any costs associated with the
preparation and filing of an I-485 with the USCIS.
付的
【在 p******l 的大作中提到】
: PERM和140是以单位名义申请的,一定需要单位给付,但涉及的律师费是可以个人支付的
: 485好像是可以个人支付
http://counsel.cua.edu/fedlaw/lpr.cfm
1. PERM (Labor Certification Application): Under the DOL's new rules, the
employer (i.e., college) must bear the costs associated with the preparation
and filing of a PERM application with the DOL including, but not limited to
, the advertising costs and attorney's fees (in most situations).
2. I-140 Immigration Petition for Alien Worker: There is no legal obligation
for the employer to pay any costs associated with the preparation and
filing of an I-140 with the USCIS.
3. I-485 Application to Adjust Status to Permanent Residence: There is no
legal obligation for the employer to pay any costs associated with the
preparation and filing of an I-485 with the USCIS.
付的
【在 p******l 的大作中提到】
: PERM和140是以单位名义申请的,一定需要单位给付,但涉及的律师费是可以个人支付的
: 485好像是可以个人支付
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