z*a
2 楼
I am running a 5% SDS gel.
When started running for ~1 hr, I set it at low voltage (60v) and went out
for something to eat. About 3 hrs
later I was back and found out that I did not press start button. The
Bromophenol blue band is blurred and
about 1 cm in width.
I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
normal with my eyeballs.
Is it still good and worth continuing?
Thanks
When started running for ~1 hr, I set it at low voltage (60v) and went out
for something to eat. About 3 hrs
later I was back and found out that I did not press start button. The
Bromophenol blue band is blurred and
about 1 cm in width.
I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
normal with my eyeballs.
Is it still good and worth continuing?
Thanks
g*t
3 楼
make sense
我屯了黄金，准备度假了
【在 p***r 的大作中提到】
: 我估计2969起来的这波三十分钟向上在上周走出个中枢之后，基本快要走死了。
我屯了黄金，准备度假了
【在 p***r 的大作中提到】
: 我估计2969起来的这波三十分钟向上在上周走出个中枢之后，基本快要走死了。
z*a
4 楼
ding yi xia
any comments?
any comments?
i*l
5 楼
go ahead, it should be no rpoblem.
You are lucky that you forgot pressing start button, otherwise 5% gel with
over 3h running, a lot of proteins will run out.
Bromophenol blue bands are all fine like
【在 z*******a 的大作中提到】
: I am running a 5% SDS gel.
: When started running for ~1 hr, I set it at low voltage (60v) and went out
: for something to eat. About 3 hrs
: later I was back and found out that I did not press start button. The
: Bromophenol blue band is blurred and
: about 1 cm in width.
: I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
: normal with my eyeballs.
: Is it still good and worth continuing?
: Thanks
You are lucky that you forgot pressing start button, otherwise 5% gel with
over 3h running, a lot of proteins will run out.
Bromophenol blue bands are all fine like
【在 z*******a 的大作中提到】
: I am running a 5% SDS gel.
: When started running for ~1 hr, I set it at low voltage (60v) and went out
: for something to eat. About 3 hrs
: later I was back and found out that I did not press start button. The
: Bromophenol blue band is blurred and
: about 1 cm in width.
: I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
: normal with my eyeballs.
: Is it still good and worth continuing?
: Thanks
z*a
6 楼
Thank you, ildjlll.
g*y
7 楼
1。 查你的gel是不是放反了，2。查你的running buffer是不是正确。
Bromophenol blue bands are all fine
like
【在 z*******a 的大作中提到】
: I am running a 5% SDS gel.
: When started running for ~1 hr, I set it at low voltage (60v) and went out
: for something to eat. About 3 hrs
: later I was back and found out that I did not press start button. The
: Bromophenol blue band is blurred and
: about 1 cm in width.
: I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
: normal with my eyeballs.
: Is it still good and worth continuing?
: Thanks
Bromophenol blue bands are all fine
like
【在 z*******a 的大作中提到】
: I am running a 5% SDS gel.
: When started running for ~1 hr, I set it at low voltage (60v) and went out
: for something to eat. About 3 hrs
: later I was back and found out that I did not press start button. The
: Bromophenol blue band is blurred and
: about 1 cm in width.
: I started the run again. Now the stained protein marker bands and the Bromophenol blue bands are all fine like
: normal with my eyeballs.
: Is it still good and worth continuing?
: Thanks
i*f
8 楼
I think it's going to be okay
c*y
9 楼
为啥楼上回帖的标题是那个？
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