BCL6 leukemia study appeared in Nature - 未名空间MITBBS历史存档

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BCL6 leukemia study appeared in Nature

BCL6 leukemia study appeared in Nature# Biology - 生物学

l*22011-06-10 07:06

1 楼

哈工大EE毕业,西安科技EE硕士,6年工作经验,嵌入式软件工程师,编程
能力极强,制作了几个app store里的app 包括 App PalmGo 行人导航掌城路况通百度
路况
本人对学术研究很有热情,很希望来美国读书,弱项就是本科成绩不高(74),哈工大一般
给分普遍低,研究生成绩80多分,请问这种情况申请博士有希望吗,如果博士不成,申请硕
士有把握吗,肯请各位老师前辈给下选校/申请建议,哪些学校对本科GPA不太看重,注重
工作经历,以便有的放矢.
请有兴趣的联系,可以发送详细简历.
多谢!!!

c*22011-06-10 07:06

2 楼

I have been struggling in understanding the results of the supplementary
figure 6 [ http://www.nature.com/nature/journal/v473/n7347/extref/nature09883-s1.pdf ]. The Bcl6 -/- strains was shown to have higher expression of Bcl6 than in the wild type. How could this happen? I kind of think it should be the opposite.
Any clues?

e*g2011-06-10 07:06

3 楼

硕士没事，
如果只有编程能力强，那比较麻烦，

【在 l*****2 的大作中提到】

: 哈工大EE毕业,西安科技EE硕士,6年工作经验,嵌入式软件工程师,编程
: 能力极强,制作了几个app store里的app 包括 App PalmGo 行人导航掌城路况通百度
: 路况
: 本人对学术研究很有热情,很希望来美国读书,弱项就是本科成绩不高(74),哈工大一般
: 给分普遍低,研究生成绩80多分,请问这种情况申请博士有希望吗,如果博士不成,申请硕
: 士有把握吗,肯请各位老师前辈给下选校/申请建议,哪些学校对本科GPA不太看重,注重
: 工作经历,以便有的放矢.
: 请有兴趣的联系,可以发送详细简历.
: 多谢!!!

o*r2011-06-10 07:06

4 楼

someone has mentioned before that in KO, not the whole gene was completely
deleted.
In most cases, just an exon. So depends on which part of the gene been
probed in microarray,
you might see higher level profile because cells tend to increase the gene
expression level trying to
compromise the KO defect, though not effective.

strains was shown to have higher expression of Bcl6 than in the wild type.
How could this happen? I kind of
think it should be the opposite.

【在 c*2 的大作中提到】

: I have been struggling in understanding the results of the supplementary
: figure 6 [ http://www.nature.com/nature/journal/v473/n7347/extref/nature09883-s1.pdf ]. The Bcl6 -/- strains was shown to have higher expression of Bcl6 than in the wild type. How could this happen? I kind of think it should be the opposite.
: Any clues?

l*o2011-06-10 07:06

5 楼

我还不是professor。
但是我当时有相同的情况，我硕士毕业工作5年才申请的。我也是EE的背景，但出了校
门一直做计算机。申请时，我主要和那些导师先联系，附上自己的简历和一个非常粗浅
的所谓Paper。
如果有些导师回信，我再试图每一二个月Update自己的新情况，比如投了一些专利啊什
么的。从回信的导师中，我试图跳出一些学校申请。最后，拿了几个AD和两个Offer。
Point是一定要找对自己背景感兴趣的老师。很多top20以上的学校也许不是非常重视
GPA的。而且，你同学的背景可以试一下计算机的系统方向和编程语言方向。
另外，一些学校也找一些计算机硕士，比如哥伦比亚，USC和UMASS等。有工作经验是个
优势。可以等过来后再申请钱。

c*22011-06-10 07:06

6 楼

Hi oranger,
Thanks for the explanation. It is helpful.
I actually downloaded and checked the expression data. Actually there are
two probe sets targeting the Bcl6, one of which as expected has low
expressoin of bcl6 in the mutant strain compared to the wild type.
Interestingly I also noticed that the Trp53 gene has the opposite gene
expression levels as shown in that figure too. It is really confusing.
Thanks.

【在 o*****r 的大作中提到】

: someone has mentioned before that in KO, not the whole gene was completely
: deleted.
: In most cases, just an exon. So depends on which part of the gene been
: probed in microarray,
: you might see higher level profile because cells tend to increase the gene
: expression level trying to
: compromise the KO defect, though not effective.
:
: strains was shown to have higher expression of Bcl6 than in the wild type.
: How could this happen? I kind of

o*r2011-06-10 07:06

7 楼

I don't know about that.
You could always email the authors for clarification.

【在 c*2 的大作中提到】

: Hi oranger,
: Thanks for the explanation. It is helpful.
: I actually downloaded and checked the expression data. Actually there are
: two probe sets targeting the Bcl6, one of which as expected has low
: expressoin of bcl6 in the mutant strain compared to the wild type.
: Interestingly I also noticed that the Trp53 gene has the opposite gene
: expression levels as shown in that figure too. It is really confusing.
: Thanks.

c*22011-06-10 07:06

8 楼

Just a follow-up on this. In case some of you are interested. I emailed the
author and here is his response.
==
Thanks for your interest and your question. We made the same observation as
you did. Trp53 is represented by multiple probe sets (1438808_at, 1438542_at
, 1459780_at, 1457623_x_at, 1427739_a_at, 1426538_a_at) and only one of them
shows indeed upregulation in Imatinib-treated BCL6-/- leukemia cells (i.e.
the one you can see in Figure S6). Figure S6 shows examples of microarray
probe sets with BCL6-dependent gene expression changes.
However, our conclusion regarding BCL6-dependent regulation of p53 is not
based on these microarray data. Using various ChIP techniques, we could show
that BCL6 is strongly recruited to the BCL6 promoter. More importantly, our
Western blots show that BCL6-/- leukemia cells express extremely high
levels of p53 protein compared to BCL6-wildtype leukemia cells. Likewise,
qRT-PCR data show that mRNA levels of p53 are much higher in BCL6-/- cells.
We often see that microarray data not always correctly predict real gene
expression changes. For this reason, we would not trust microarray data per
se, unless we can verify them by qRT-PCR and/or Western blot or Flow
cytometry.
Please let me know if you have other questions or if we can help otherwise
with your experiments on BCL6/p53.
Kind regards,
Markus

【在 o*****r 的大作中提到】

: I don't know about that.
: You could always email the authors for clarification.