C*b
5 楼
We have a RNAi screener who spent around 3 months to optimize the MEF
transfection condition. Finally she got 70~80% transfection efficiency.
Summary
Reagent: DharmaFECT
Cell: 30% confluent MEF instead of 70~80% confluent
Good luck!
transfection condition. Finally she got 70~80% transfection efficiency.
Summary
Reagent: DharmaFECT
Cell: 30% confluent MEF instead of 70~80% confluent
Good luck!
x*y
8 楼
请教有经验的大侠:
想在MEF细胞里,做转染。哪个试剂效率高啊?另外,用病毒转染,效率能有多少?
想在MEF细胞里,做转染。哪个试剂效率高啊?另外,用病毒转染,效率能有多少?
C*b
12 楼
We have a RNAi screener who spent around 3 months to optimize the MEF
transfection condition. Finally she got 70~80% transfection efficiency.
Summary
Reagent: DharmaFECT
Cell: 30% confluent MEF instead of 70~80% confluent
Good luck!
transfection condition. Finally she got 70~80% transfection efficiency.
Summary
Reagent: DharmaFECT
Cell: 30% confluent MEF instead of 70~80% confluent
Good luck!
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