b*c
2 楼
养细胞只信任corning
s*o
3 楼
I believe you have to test by yourself.
here is my story with Corning....
I initially used corning for my thesis research simply because corning was
the brand everyone else was using....and when I started my Flex assays, our
group then consumed at least 10 Corning plates per week. It was all working
well until three year later when I myself became a senior member ( PI lost
HHMI and all PostDoc except one left for better positions)....
starting from a day I could never remember, Flex assay just didn't work
anymore. under microscope 293 cells seemed fine with the right confluence.
The only PostDoc and I started the painful process of trouble shooting....
that was the most painful 6 months I had during gradschool... I almost
looked into everything from host cells to DNA to transfectant....
until one night when I held the plate without glove, I suddenly felt dust-
like stuff under corning plate.....with that clue and more efforts, I
finally found out Corning plate was in fact contaminated by some kind of
fungus....Ironically the fungus grows just slow enough so that 293 cells
could still form confluence, but powerful enough to kill our assay....
I then switched to BD 96 well plates, and was able to finish my project.
Even today, I still believe such experience with Corning really scared me
away from research.
here is my story with Corning....
I initially used corning for my thesis research simply because corning was
the brand everyone else was using....and when I started my Flex assays, our
group then consumed at least 10 Corning plates per week. It was all working
well until three year later when I myself became a senior member ( PI lost
HHMI and all PostDoc except one left for better positions)....
starting from a day I could never remember, Flex assay just didn't work
anymore. under microscope 293 cells seemed fine with the right confluence.
The only PostDoc and I started the painful process of trouble shooting....
that was the most painful 6 months I had during gradschool... I almost
looked into everything from host cells to DNA to transfectant....
until one night when I held the plate without glove, I suddenly felt dust-
like stuff under corning plate.....with that clue and more efforts, I
finally found out Corning plate was in fact contaminated by some kind of
fungus....Ironically the fungus grows just slow enough so that 293 cells
could still form confluence, but powerful enough to kill our assay....
I then switched to BD 96 well plates, and was able to finish my project.
Even today, I still believe such experience with Corning really scared me
away from research.
相关阅读
刚刚在lab meeting上吵架了authorship的总结及其他一些感想杀老板----版上有人想过么牛人美女老闫近况，很拼命啊 (转载)Arabidopsis interactome map有谁最近看过让人眼前一亮的文章没有？怎么从芯片数据中挑出我要的200个gene的数据进行分析？怎么查DOE的FUNDINGNYC有那些做病毒的大牛呢How to explain real-time false positive problem?康乃尔大学中国禽兽不如教授 (转载)关于cre x flox 老鼠的鉴定一个质粒上可以同时存在两个ori吗？想付诸法律得到赔偿，各位给个看法装B的老板SV129/C57 albino 后代的奇怪 Phenotype有没有人做western用Biorad的半干转移，给点指示吧我只有抗体如何做ELISA?food science现在情况如何