Re: 超声波裂解E.coli细胞求救！# Biology - 生物学
w*r
1 楼
stir well, dip the tip fully in the cell suspend, use medium output,
a little foam is ok, don't be too nervous. I have done it hundreds of
times, protein is much tougher than you thing.
if there is no activity, it means there is not, not because of
the sonication. By the way, e coli is not that tough, don't sonicate too
long, and keep all your stuff in ice during the whole procedure. As of my
experience, heating is a bigger problem than foam.
a little foam is ok, don't be too nervous. I have done it hundreds of
times, protein is much tougher than you thing.
if there is no activity, it means there is not, not because of
the sonication. By the way, e coli is not that tough, don't sonicate too
long, and keep all your stuff in ice during the whole procedure. As of my
experience, heating is a bigger problem than foam.