Re: PCR产物酶切一问# Biology - 生物学
c*A
1 楼
first of all, everything could happen in biology.. hehe..
so, for trouble shooting:
1. run gel to check your sample before digestion but after
purification.
OK --> digestion problem
smear --> purification problem.
2. if purification problem, try to use a PCR purification kit.
3. if digestion problem.
A. make sure the fragment you cloned did not contain any
HindIII/EcoRI
B. try a sequencial digestion..
4. just a reminder:
HindIII will have prolbem to cut when your si
so, for trouble shooting:
1. run gel to check your sample before digestion but after
purification.
OK --> digestion problem
smear --> purification problem.
2. if purification problem, try to use a PCR purification kit.
3. if digestion problem.
A. make sure the fragment you cloned did not contain any
HindIII/EcoRI
B. try a sequencial digestion..
4. just a reminder:
HindIII will have prolbem to cut when your si