Re: A question on PCR efficiency# Biology - 生物学
m*n
1 楼
everything you mentioned is considered, good comments, esp the last one
for example, unknown SNP/mutation in your primer region will
certainly change PCR efficiency. Or DNA modification might change
primer binding. If you are a biologist, you would think of these first.
DNA 2nd structure is certainly another thing to consider, esp. since my
amplicons are short (~70-150 bp). My student spent quite some time on
this aspect (quite some $ on software as well) and results are dubious.
For DNA 2nd str
for example, unknown SNP/mutation in your primer region will
certainly change PCR efficiency. Or DNA modification might change
primer binding. If you are a biologist, you would think of these first.
DNA 2nd structure is certainly another thing to consider, esp. since my
amplicons are short (~70-150 bp). My student spent quite some time on
this aspect (quite some $ on software as well) and results are dubious.
For DNA 2nd str
