c*u
2 楼
每部电视剧前边都要放个没停
x*m
3 楼
如果想从epigenomics的角度来研究human ips和es的不同,是不是很难?
问过一个教授,他说,要做人的话,必须ips和es来自于同一个人,这样才能排除
genomic DNA的variation,但这是不可能的,人取了es就不可能有ips了。
所以他说只能做mouse的stem cell。找同一strain的mouse,去es,再用somatic 诱导
出ips,这样比较他们的不同才有意义。
大家说说看,这种说法对吗?
问过一个教授,他说,要做人的话,必须ips和es来自于同一个人,这样才能排除
genomic DNA的variation,但这是不可能的,人取了es就不可能有ips了。
所以他说只能做mouse的stem cell。找同一strain的mouse,去es,再用somatic 诱导
出ips,这样比较他们的不同才有意义。
大家说说看,这种说法对吗?
t*n
5 楼
我的也是,优酷土豆都这样
a*a
6 楼
同一strain的老鼠也有variation啊
我当时的想法是建一个es系、挑单克隆出来一半存了,
另一半诱导分化成终末分化细胞然后再ips
不过中间还有好几个技术问题
而且epigenome variation必然很大。。
我当时的想法是建一个es系、挑单克隆出来一半存了,
另一半诱导分化成终末分化细胞然后再ips
不过中间还有好几个技术问题
而且epigenome variation必然很大。。
r*f
7 楼
跟Jude law那会就长得一搬
过了这么多年了
过了这么多年了
T*i
8 楼
搜狐也是。明显是花大钱强退新剧
x*m
9 楼
多谢回复,中间好几个技术问题是什么?
我不是做stem cell,多谢指教
我不是做stem cell,多谢指教
n*k
11 楼
what are you thinking? from the way you asked, I am not sure you would want(
you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
tons of people are doing/thinking this kind of stuff if you are thinking of
what I am referring to here...I am not sure there is any valid approach,
nor valid significance...and then future continuation?
【在 x******m 的大作中提到】
: 如果想从epigenomics的角度来研究human ips和es的不同,是不是很难?
: 问过一个教授,他说,要做人的话,必须ips和es来自于同一个人,这样才能排除
: genomic DNA的variation,但这是不可能的,人取了es就不可能有ips了。
: 所以他说只能做mouse的stem cell。找同一strain的mouse,去es,再用somatic 诱导
: 出ips,这样比较他们的不同才有意义。
: 大家说说看,这种说法对吗?
you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
tons of people are doing/thinking this kind of stuff if you are thinking of
what I am referring to here...I am not sure there is any valid approach,
nor valid significance...and then future continuation?
【在 x******m 的大作中提到】
: 如果想从epigenomics的角度来研究human ips和es的不同,是不是很难?
: 问过一个教授,他说,要做人的话,必须ips和es来自于同一个人,这样才能排除
: genomic DNA的variation,但这是不可能的,人取了es就不可能有ips了。
: 所以他说只能做mouse的stem cell。找同一strain的mouse,去es,再用somatic 诱导
: 出ips,这样比较他们的不同才有意义。
: 大家说说看,这种说法对吗?
x*m
12 楼
你的英文。。。
我看了半天才懂你的意思,sorry。
我是做bioinfo的,也没想跳这个领域,只是在做数据分析的时候,跟做这个方向的教
授聊过,很好奇就上来问问。
want(
BTW,
of
【在 n********k 的大作中提到】
: what are you thinking? from the way you asked, I am not sure you would want(
: you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
: tons of people are doing/thinking this kind of stuff if you are thinking of
: what I am referring to here...I am not sure there is any valid approach,
: nor valid significance...and then future continuation?
我看了半天才懂你的意思,sorry。
我是做bioinfo的,也没想跳这个领域,只是在做数据分析的时候,跟做这个方向的教
授聊过,很好奇就上来问问。
want(
BTW,
of
【在 n********k 的大作中提到】
: what are you thinking? from the way you asked, I am not sure you would want(
: you are not ready, I mean, sorry) to jump into this hole/compitition...BTW,
: tons of people are doing/thinking this kind of stuff if you are thinking of
: what I am referring to here...I am not sure there is any valid approach,
: nor valid significance...and then future continuation?
n*k
13 楼
Good for you...as long as you figured it out, I am not gonna be sorry for my
english...BTW, I take no shame on my English no matter how you folks bash
me...I would have no time to do any experiments if I had to water either in
Chinese like Sunny or perfect English like Dr. Biologist...I am just
nevertthink and I don't care...this would be a first time and hopefully a
last time I would ever explain on my "wonderful" english
【在 x******m 的大作中提到】
: 你的英文。。。
: 我看了半天才懂你的意思,sorry。
: 我是做bioinfo的,也没想跳这个领域,只是在做数据分析的时候,跟做这个方向的教
: 授聊过,很好奇就上来问问。
:
: want(
: BTW,
: of
english...BTW, I take no shame on my English no matter how you folks bash
me...I would have no time to do any experiments if I had to water either in
Chinese like Sunny or perfect English like Dr. Biologist...I am just
nevertthink and I don't care...this would be a first time and hopefully a
last time I would ever explain on my "wonderful" english
【在 x******m 的大作中提到】
: 你的英文。。。
: 我看了半天才懂你的意思,sorry。
: 我是做bioinfo的,也没想跳这个领域,只是在做数据分析的时候,跟做这个方向的教
: 授聊过,很好奇就上来问问。
:
: want(
: BTW,
: of
M*n
14 楼
your English is OK, at least I can understand most of it.
a little suggestion, try to use less "would" or ..., coz, you talk like that
British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
a little suggestion, try to use less "would" or ..., coz, you talk like that
British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
n*k
15 楼
alright alright...I would have killed you,,,,,it is a secret,,,you know,,,,
hoho
that
movie"King's Speech". )
【在 M*****n 的大作中提到】
: your English is OK, at least I can understand most of it.
: a little suggestion, try to use less "would" or ..., coz, you talk like that
: British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
:
: my
: in
hoho
that
movie"King's Speech". )
【在 M*****n 的大作中提到】
: your English is OK, at least I can understand most of it.
: a little suggestion, try to use less "would" or ..., coz, you talk like that
: British King George V (or VI, I don't remember, you know what I mean, the movie"King's Speech". )
:
: my
: in
m*5
16 楼
Lz is thinking in the absolutely right direction.... too bad there are at
least 2 nature cell
biology paper published in this topic
least 2 nature cell
biology paper published in this topic
n*k
17 楼
this is virtually no brain research in the field, check this history of
nuclear cloning, that's exactly what has happened there---Cool, equvilent of
ES---ops--they are different... Those studies are more for publications and
news as far as I am concerned...I don't mean they have no values though...
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
nuclear cloning, that's exactly what has happened there---Cool, equvilent of
ES---ops--they are different... Those studies are more for publications and
news as far as I am concerned...I don't mean they have no values though...
【在 m******5 的大作中提到】
: Lz is thinking in the absolutely right direction.... too bad there are at
: least 2 nature cell
: biology paper published in this topic
j*x
18 楼
a little bit over reacting ba...
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
my
in
【在 n********k 的大作中提到】
: Good for you...as long as you figured it out, I am not gonna be sorry for my
: english...BTW, I take no shame on my English no matter how you folks bash
: me...I would have no time to do any experiments if I had to water either in
: Chinese like Sunny or perfect English like Dr. Biologist...I am just
: nevertthink and I don't care...this would be a first time and hopefully a
: last time I would ever explain on my "wonderful" english
n*k
25 楼
there was too come out the same time I believe...both from harvard? and
there was tons of news too, i don't know for what? the same old stories on
news again, or what? many experts were praising its a big deal too but do
they really buy that? they know better than that at least from the nuclear
cloning lessons...
【在 M****7 的大作中提到】
: GQ Daley 2010 "Epigenetic memory in induced pluripotent
: stem cells"
there was tons of news too, i don't know for what? the same old stories on
news again, or what? many experts were praising its a big deal too but do
they really buy that? they know better than that at least from the nuclear
cloning lessons...
【在 M****7 的大作中提到】
: GQ Daley 2010 "Epigenetic memory in induced pluripotent
: stem cells"
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