Re: Help! Qualify Question# Biology - 生物学
s*y
1 楼
FPLC or HPLC Gel filtration column, viscosity assay or sedimentation velocity
assay can be used for dimer/polymer assay. Native gel is also used for such
purpose. SDS-PAGE is NOT!
Antibody? Buy it from commercial company.
Actually, for your question, you DON"T have to do those dimer things at all.
They are the properties of a protein but has NOTHING to do with purity.
A proper folding is needed, if your lab is gonna to test it's activity.
However,if only the protein sequence is corncerned (e.g.,
assay can be used for dimer/polymer assay. Native gel is also used for such
purpose. SDS-PAGE is NOT!
Antibody? Buy it from commercial company.
Actually, for your question, you DON"T have to do those dimer things at all.
They are the properties of a protein but has NOTHING to do with purity.
A proper folding is needed, if your lab is gonna to test it's activity.
However,if only the protein sequence is corncerned (e.g.,